What do we have for obtaining information about the direction of a biochemical reaction?

Analysis of the biochemical complex N1

We investigate how the physical parameters characterizing system N1 (wtBRAF) change when the system transitions to state N6 and N3 (mutBRAF)

Each protein complex has its own physical parameters that determine the interaction:

The analysis of changes in these values makes it possible to detect the formation and decay of biological complexes, as well as to rank biological complexes according to their degree of affinity.